The study presents a comparative in vitro assessment of the biological efficacy of chemical and microbiological fungicides against five pathogens causing wood diseases in fruit crops, isolated in Crimea, Krasnodar Krai, and the Moscow Region. The research objects were pure cultures of Cytospora leucostoma, Diaporthe eres, Didymella macrostoma, Diplodia seriata, and Seimatosporium lichenicola, identified by ITS region sequencing. The efficacy of Insignia (cyprodinil + fludioxonil), Kapella (propiconazole + flutriafol + difenoconazole), Riviera (pyraclostrobin + tebuconazole + difenoconazole), and Stakkato (Bacillus amyloliquefaciens 133) was evaluated using the agar block application method, with biological efficacy calculated according to Abbott’s formula. It was found that Insignia provided 100 % growth inhibition of C. leucostoma and S. lichenicola, 89.1 % of Diaporthe eres, and 90.7 % of Didymella macrostoma. Kapella and Riviera demonstrated 73–94 % efficacy against all pathogens, including 82.6 % and 82.8 % inhibition of Diplodia seriata. The biopreparation Stakkato showed 100 % efficacy against C. leucostoma and Diaporthe eres, 83.1 % against Didymella macrostoma, and 67.1 % against S. lichenicola. The results confirm the high efficacy of combined fungicides and the potential of the B. amyloliquefaciens 133 strain as an alternative to chemical agents, while the antagonistic effect of the biopreparation is shown to be species‑specific.
Cytospora leucostoma, Diaporthe eres, Didymella macrostoma, Diplodia seriata, Seimatosporium lichenicola, Insignia, Kapella, Riviera, Staccato



